Molecular Primer Pairs
Analyte Specific Reagents (ASRs) for use in Laboratory Developed Tests (LDTs)
Diasorin has an extensive and growing menu of over 65 Analyte Specific Reagents* (ASRs) for the amplification and detection of relevant viral, bacterial, fungal and parasitic targets as well as common genetic single-nucleotide polymorphisms.
*Analytical and performance characteristics are not established.
Viral
Product |
Description |
Code |
Volume/Quantity/Reactions |
|---|---|---|---|
| ADENOVIRUS 3′ HEXON PRIMER PAIR |
For amplification and detection of the 3′ region of the gene for adenovirus hexon polypeptide with a FAM labeled forward primer. |
MOL9011 MOL9311 |
50 µL 500 µL |
| ADENOVIRUS 5′ HEXON PRIMER PAIR |
For amplification and detection of the 5′ region of the gene for adenovirus hexon polypeptide with a FAM labeled forward primer. |
MOL9012 MOL9312 |
50 µL 500 µL |
| BKV GEN II PRIMER PAIR |
For amplification and detection of a well conserved region of the VP2-VP3-VP1 genes of BK polyomavirus with a FAM labeled probe, forward primer, and reverse primer. |
MOL9069 |
25 µL |
| BKV PRIMER PAIR |
For amplification and detection of the 5′ region of the VP2 gene with a FAM labeled forward primer. |
MOL9009 |
50 µL |
| CHIKUNGUNYA PRIMER PAIR |
For amplification and detection of the Chikungunya virus Alpha E1 Glycoprotein gene with a FAM labeled forward primer. |
MOL9039 |
50 µL |
| CMV PRIMER PAIR |
For amplification and detection of the CMV UL83 gene with a FAM labeled forward primer. |
MOL9002 |
50 µL |
| DENGUE VIRUS 1 PRIMER PAIR |
For amplification and detection of the NS5 gene with a FAM labeled forward primer. |
MOL9041 |
50 µL |
| DENGUE VIRUS 2 PRIMER PAIR |
For amplification and detection of the NS3 gene with a CFR610 labeled forward primer. |
MOL9042 |
50 µL |
| DENGUE VIRUS 3 PRIMER PAIR |
For amplification and detection of the NS5 gene with a FAM labeled forward primer. |
MOL9043 |
50 µL |
| DENGUE VIRUS 4 PRIMER PAIR |
For amplification and detection of the capsid gene with a CFR610 labeled forward primer. |
MOL9044 |
50 µL |
| EBV PRIMER PAIR |
For amplification and detection of the LMP2A gene with a FAM labeled forward primer. |
MOL9008 |
50 µL |
| ENTEROVIRUS PRIMER PAIR |
For amplification and detection of the 5′ non-coding region of the enterovirus genome with a FAM labeled forward primer. |
MOL9020 |
50 µL |
| HHV-6 (HUMAN HERPESVIRUS 6) PRIMER PAIR |
For amplification and detection of the P100 gene with a FAM labeled forward primer. |
MOL9022 |
50 µL |
| HHV-8 PRIMER PAIR |
For amplification and detection of a well conserved region of the ORF26 gene of human herpesvirus 8 with a FAM labeled probe, forward primer, and reverse primer. |
MOL9099 |
25 μL |
| HMPV (HUMAN METAPNEUMOVIRUS) PRIMER PAIR |
For amplification and detection of a well conserved region of the F gene for hMPV with FAM labeled forward primer. |
MOL9029 |
50 µL |
| HSV-1 (HERPES SIMPLEX VIRUS TYPE 1) GEN II PRIMER PAIR |
For amplification and detection of the HSV-1 DNA polymerase gene with a CAL Fluor Red 610 labeled forward primer. |
MOL9037 MOL9337 |
50 µL 200 µL |
| HSV-2 (HERPES SIMPLEX VIRUS TYPE 2) PRIMER PAIR |
For amplification and detection of the DNA polymerase gene with a FAM labeled forward primer. |
MOL9004 MOL9304 |
50 µL 200 µL |
| INFLUENZA A PRIMER PAIR |
For amplification and detection of the Matrix gene with a FAM labeled forward primer. |
MOL9016 |
50 µL |
| INFLUENZA B PRIMER PAIR |
For amplification and detection of the Matrix gene with a JOE labeled forward primer. |
MOL9017 |
50 µL |
| JCV GEN II PRIMER PAIR |
For amplification and detection of a well conserved region of the VP1-VP2-VP3 gene of JC polyomavirus with a JOE labeled probe, forward primer, and reverse primer. |
MOL9081 |
25 μL |
| JCV PRIMER PAIR |
For amplification and detection of the VP2/3 gene with a CFR610 labeled forward primer. |
MOL9021 MOL9321 |
50 µL 500 µL |
| MONKEYPOX (MPX) PRIMER PAIR |
For amplification and detection of a conserved region of the B17R/B18R gene for monkeypox virus clades with a JOE labeled forward primer. |
MOL9202 |
50 μL |
| NOROVIRUS I PRIMER PAIR |
For amplification and detection of a well conserved region between ORF1 and ORF2 of the norovirus genogroup I with a CFR610 labeled forward primer. |
MOL9027 |
50 µL |
| NOROVIRUS II PRIMER PAIR |
For amplification and detection of a well conserved region between ORF1 and ORF2 of the norovirus genogroup II with a FAM labeled forward primer. |
MOL9028 |
50 µL |
| PARAINFLUENZA 1 PRIMER PAIR |
For amplification and detection of the hemagglutinin-neuraminidase (HN) gene with a FAM labeled forward primer. |
MOL9030 |
50 µL |
| PARAINFLUENZA 2 PRIMER PAIR |
For amplification and detection of the hemagglutinin-neuraminidase (HN) gene with a JOE labeled forward primer. |
MOL9031 |
50 µL |
| PARAINFLUENZA 3 PRIMER PAIR |
For amplification and detection of the hemagglutinin-neuraminidase (HN) gene with a CFR610 labeled forward primer. |
MOL9032 |
50 µL |
| PARECHOVIRUS |
For amplification and detection of the 5′ untranslated region of the gene with a CFR610 labeled forward primer. |
MOL9038 |
50 µL |
| PARVOVIRUS PRIMER PAIR |
For amplification and detection of the VP1 gene with a FAM labeled forward primer. |
MOL9019 MOL9319 |
50 µL 500 µL |
| RSV (RESPIRATORY SYNCYTIAL VIRUS) PRIMER PAIR |
For amplification and detection of the Matrix gene with a CAL Fluor Red 610 labeled forward primer. |
MOL9014 |
50 µL |
| VZV PRIMER PAIR |
For amplification and detection of the DNA polymerase gene of varicella zoster virus labeled with a JOE labeled forward primer. |
MOL9024 MOL9324 |
50 µL 200 µL |
Genetics
Product |
Description |
Code |
Volume/Quantity/Reactions |
|---|---|---|---|
| FACTOR V LEIDEN HYBEACON GEN II |
For amplification and detection of the sequence that surrounds nucleotide 1691 of the human factor V gene with a Q670 labeled probe, forward and reverse primers. |
MOL9771 |
100 µL |
| FACTOR V LEIDEN MUTANT PRIMER PAIR |
For amplification and detection of the Leiden (G1691A) mutation with a CFR610 labeled forward primer. |
MOL9050 |
50 µL |
| FACTOR V WILD TYPE PRIMER PAIR |
For amplification and detection of the wild type sequence surrounding nucleotide 1691 with a FAM labeled forward primer. |
MOL9051 |
50 µL |
| MTHFR 677 HYBEACON GEN II |
For amplification and detection of the sequence that surrounds nucleotide 677 of the human methylenetetrahydrofolate reductase gene with a CFR610 labeled probe, forward and reverse primers. |
MOL9775 |
100 µL |
| MTHFR 677 MUTANT PRIMER PAIR |
For amplification and detection of the C677T mutation with a CFR610 labeled forward primer. |
MOL9054 |
50 µL |
| MTHFR 677 WILD TYPE |
For amplification and detection of the wild type sequence surrounding nucleotide 677 with a FAM labeled forward primer. |
MOL9055 |
50 µL |
| MTHFR 1298 HYBEACON GEN II |
For amplification and detection of the sequence that surrounds nucleotide 1298 of the human methylenetetrahydrofolate reductase gene with a Q670 labeled probe, forward and reverse primers. |
MOL9777 |
100 µL |
| MTHFR 1298 MUTANT PRIMER PAIR |
For amplification and detection of the 1298C mutation with a Quasar 670 labeled forward primer. |
MOL9056 |
50 µL |
| MTHFR 1298 WILD TYPE PRIMER PAIR |
For amplification and detection of the wild type sequence surrounding nucleotide 1298 with a JOE labeled forward primer. |
MOL9057 |
50 µL |
| PROTHROMBIN (FII) HYBEACON GEN II |
For amplification and detection of the sequence that surrounds nucleotide 20210 of the human prothrombin (factor II) gene with a CFR610 labeled probe, forward and reverse primers. |
MOL9773 |
100 µL |
| PROTHROMBIN (FII) MUTANT PRIMER PAIR |
For amplification and detection of the Prothrombin (Factor II) G20210A mutation with a Quasar 670 labeled forward primer. |
MOL9052 |
50 µL |
| PROTHROMBIN (FII) WILD TYPE PRIMER PAIR |
For amplification and detection of the Prothrombin (Factor II) wild type sequence surrounding nucleotide 20210 with a JOE labeled forward primer. |
MOL9053 |
50 µL |
Parasite
Product |
Description |
Code |
Volume/Quantity/Reactions |
|---|---|---|---|
| PLASMODIUM PRIMER PAIR |
For amplification and detection of a well conserved region of the mitochondrial cytochrome c oxidase III (cox3) gene for Plasmodium species with a FAM labeled probe, forward primer, and reverse primer. |
MOL9065 |
25 µL |
| T. GONDII PRIMER PAIR |
For amplification and detection of the B1 gene with a FAM labeled forward primer. |
MOL9025 |
50 µL |
Fungal
Product |
Description |
Code |
Volume/Quantity/Reactions |
|---|---|---|---|
| C. AURIS PRIMER PAIR |
For amplification and detection of a well conserved region of the internal transcribed spacer 2 (ITS2) region in the rRNA gene for Candida auris with a FAM labeled probe, forward and reverse primer. |
MOL9059 |
25 μL |
| P. JIROVECII PRIMER PAIR |
For amplification and detection of the mtLSU gene with a CFR 610 labeled forward primer. |
MOL9098 |
25 µL |
Bacterial
Product |
Description |
Code |
Volume/Quantity/Reactions |
|---|---|---|---|
| A. PHAGOCYTOPHILUM PRIMER PAIR |
For amplification and detection of the GroEL gene for Anaplasma phagocytophilum with a FAM labeled probe, forward and reverse primer. |
MOL9062 |
25 µL |
| B. HOLMESII PRIMER PAIR |
For amplification and detection of hIS1001, Bordetella holmesii insertion sequence, with a JOE labeled forward primer. |
MOL9036 |
50 µL |
| B. PARAPERTUSSIS PRIMER PAIR |
For amplification and detection of the IS1001 insertion sequence with a CAL Fluor Red 610 labeled forward primer. |
MOL9007 MOL9307 |
50 µL 200 µL |
| B. PERTUSSIS PRIMER PAIR |
For amplification and detection of the IS481 insertion sequence with a FAM labeled forward primer. |
MOL9006 MOL9306 |
50 µL 200 µL |
| BABESIA PRIMER PAIR |
For amplification and detection of a well conserved region of the 18S rRNA gene for Babesia species with a JOE labeled probe, forward and reverse primer. |
MOL9063 |
25 µL |
| BARTONELLA PRIMER PAIR |
For amplification and detection of a well conserved region of the ribosomal small subunit methyltransferase H gene (rsmH) for Bartonella species with a FAM labeled probe, forward primer, and reverse primer. |
MOL9066 |
25 µL |
| BORRELIA PRIMER PAIR |
For amplification and detection of the flaA gene gene with a FAM labeled forward primer. |
MOL9015 MOL9315 |
50 µL 500 µL |
| C. DIFFICILE PRIMER PAIR |
For amplification and detection of the toxin B gene with a FAM labeled forward primer. |
MOL9026 |
50 µL |
| C. PNEUMONIAE PRIMER PAIR |
For amplification and detection of the Cpn0980 gene with a FAM labeled forward primer. |
MOL9023 |
50 µL |
| C. PNEUMONIAE II PRIMER PAIR |
For amplification and detection of the Cpn0980 gene with a JOE labeled probe, forward and reverse primer. |
MOL9400 |
25 µL |
| EHRLICHIA PRIMER PAIR |
For amplification and detection of a well conserved region of the 16S rRNA gene for Ehrlichia species with a CAL Fluor Red 610 labeled probe, forward and reverse primer. |
MOL9064 |
25 µL |
| GROUP A STREP PRIMER PAIR |
For amplification and detection of exotoxin B (spe B) with a FAM labeled forward primer. |
MOL9033 MOL9333 MOL9333-5 |
50 µL 1000 µL 5 x 1000 µL |
| GROUP B STREP PRIMER PAIR |
For amplification and detection of CFB gene with a CFR(610) labeled forward primer. |
MOL9047 |
50 µL |
| GROUP C STREP PRIMER PAIR |
For amplification and detection of CPN gene with a CFR(610) labeled forward primer. |
MOL9093 |
50 µL |
| GROUP G STREP PRIMER PAIR |
For amplification and detection of mrcB gene with a JOE (560) labeled forward primer. |
MOL9094 |
50 µL |
| KPC PRIMER PAIR |
For amplification and detection of the Klebsiella pneumoniae carbapenemase gene, also known as blaKPC, with a FAM labeled forward primer. |
MOL9035 |
50 µL |
| L. PNEUMOPHILA PRIMER PAIR |
For amplification and detection of the pmiA gene with a CFR610 labeled forward primer. |
MOL9034 |
50 µL |
| LEGIONELLA PRIMER PAIR |
For amplification and detection of a well conserved region of the 16S rRNA gene of Legionella species with a CFR610 labeled probe, forward and reverse primers. |
MOL9401 |
25 µL |
| M. PNEUMONIAE PRIMER PAIR |
For amplification and detection of the P1 gene with a FAM labeled forward primer. |
MOL9013 |
50 µL |
| M. PNEUMONIAE II PRIMER PAIR |
For amplification and detection of a well conserved region of the P1 gene for Mycoplasma pneumoniae with a FAM labeled probe, forward primer and reverse primer. |
MOL9058 |
25 µL |