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Molecular Primer Pairs

Analyte Specific Reagents (ASRs) for use in Laboratory Developed Tests (LDTs)

Diasorin has an extensive and growing menu of over 65 Analyte Specific Reagents* (ASRs) for the amplification and detection of relevant viral, bacterial, fungal and parasitic targets as well as common genetic single-nucleotide polymorphisms.

*Analytical and performance characteristics are not established.

Viral

Product
Descrizione
Codice
Volume/Quantity/Reactions
ADENOVIRUS 3′ HEXON PRIMER PAIR

For amplification and detection of the 3′ region of the gene for adenovirus hexon polypeptide with a FAM labeled forward primer.

MOL9011

MOL9311

50 µL

500 µL

ADENOVIRUS 5′ HEXON PRIMER PAIR

For amplification and detection of the 5′ region of the gene for adenovirus hexon polypeptide with a FAM labeled forward primer.

MOL9012

MOL9312

50 µL

500 µL

BKV GEN II PRIMER PAIR

For amplification and detection of a well conserved region of the VP2-VP3-VP1 genes of BK polyomavirus with a FAM labeled probe, forward primer, and reverse primer.

MOL9069

25 µL

BKV PRIMER PAIR

For amplification and detection of the 5′ region of the VP2 gene with a FAM labeled forward primer.

MOL9009

50 µL

CHIKUNGUNYA PRIMER PAIR

For amplification and detection of the Chikungunya virus Alpha E1 Glycoprotein gene with a FAM labeled forward primer.

MOL9039

50 µL

CMV PRIMER PAIR

For amplification and detection of the CMV UL83 gene with a FAM labeled forward primer.

MOL9002

50 µL

DENGUE VIRUS 1 PRIMER PAIR

For amplification and detection of the NS5 gene with a FAM labeled forward primer.

MOL9041

50 µL

DENGUE VIRUS 2 PRIMER PAIR

For amplification and detection of the NS3 gene with a CFR610 labeled forward primer.

MOL9042

50 µL

DENGUE VIRUS 3 PRIMER PAIR

For amplification and detection of the NS5 gene with a FAM labeled forward primer.

MOL9043

50 µL

DENGUE VIRUS 4 PRIMER PAIR

For amplification and detection of the capsid gene with a CFR610 labeled forward primer.

MOL9044

50 µL

EBV PRIMER PAIR

For amplification and detection of the LMP2A gene with a FAM labeled forward primer.

MOL9008

50 µL

ENTEROVIRUS PRIMER PAIR

For amplification and detection of the 5′ non-coding region of the enterovirus genome with a FAM labeled forward primer.

MOL9020

50 µL

HHV-6 (HUMAN HERPESVIRUS 6) PRIMER PAIR

For amplification and detection of the P100 gene with a FAM labeled forward primer.

MOL9022

50 µL

HHV-8 PRIMER PAIR

For amplification and detection of a well conserved region of the ORF26 gene of human herpesvirus 8 with a FAM labeled probe, forward primer, and reverse primer.

MOL9099

25 μL

HMPV (HUMAN METAPNEUMOVIRUS) PRIMER PAIR

For amplification and detection of a well conserved region of the F gene for hMPV with FAM labeled forward primer.

MOL9029

50 µL

HSV-1 (HERPES SIMPLEX VIRUS TYPE 1) GEN II PRIMER PAIR

For amplification and detection of the HSV-1 DNA polymerase gene with a CAL Fluor Red 610 labeled forward primer.

MOL9037

MOL9337

50 µL

200 µL

HSV-2 (HERPES SIMPLEX VIRUS TYPE 2) PRIMER PAIR

For amplification and detection of the DNA polymerase gene with a FAM labeled forward primer.

MOL9004

MOL9304

50 µL

200 µL

INFLUENZA A PRIMER PAIR

For amplification and detection of the Matrix gene with a FAM labeled forward primer.

MOL9016

50 µL

INFLUENZA B PRIMER PAIR

For amplification and detection of the Matrix gene with a JOE labeled forward primer.

MOL9017

50 µL

JCV GEN II PRIMER PAIR

For amplification and detection of a well conserved region of the VP1-VP2-VP3 gene of JC polyomavirus with a JOE labeled probe, forward primer, and reverse primer.

MOL9081

25 μL

JCV PRIMER PAIR

For amplification and detection of the VP2/3 gene with a CFR610 labeled forward primer.

MOL9021

MOL9321

50 µL

500 µL

MONKEYPOX (MPX) PRIMER PAIR

For amplification and detection of a conserved region of the B17R/B18R gene for monkeypox virus clades with a JOE labeled forward primer.

MOL9202

50 μL

NOROVIRUS I PRIMER PAIR

For amplification and detection of a well conserved region between ORF1 and ORF2 of the norovirus genogroup I with a CFR610 labeled forward primer.

MOL9027

50 µL

NOROVIRUS II PRIMER PAIR

For amplification and detection of a well conserved region between ORF1 and ORF2 of the norovirus genogroup II with a FAM labeled forward primer.

MOL9028

50 µL

PARAINFLUENZA 1 PRIMER PAIR

For amplification and detection of the hemagglutinin-neuraminidase (HN) gene with a FAM labeled forward primer.

MOL9030

50 µL

PARAINFLUENZA 2 PRIMER PAIR

For amplification and detection of the hemagglutinin-neuraminidase (HN) gene with a JOE labeled forward primer.

MOL9031

50 µL

PARAINFLUENZA 3 PRIMER PAIR

For amplification and detection of the hemagglutinin-neuraminidase (HN) gene with a CFR610 labeled forward primer.

MOL9032

50 µL

PARECHOVIRUS

For amplification and detection of the 5′ untranslated region of the gene with a CFR610 labeled forward primer.

MOL9038

50 µL

PARVOVIRUS PRIMER PAIR

For amplification and detection of the VP1 gene with a FAM labeled forward primer.

MOL9019

MOL9319

50 µL

500 µL

RSV (RESPIRATORY SYNCYTIAL VIRUS) PRIMER PAIR

For amplification and detection of the Matrix gene with a CAL Fluor Red 610 labeled forward primer.

MOL9014

50 µL

VZV PRIMER PAIR

For amplification and detection of the DNA polymerase gene of varicella zoster virus labeled with a JOE labeled forward primer.

MOL9024

MOL9324

50 µL

200 µL

Genetics

Product
Descrizione
Codice
Volume/Quantity/Reactions
FACTOR V LEIDEN HYBEACON GEN II

For amplification and detection of the sequence that surrounds nucleotide 1691 of the human factor V gene with a Q670 labeled probe, forward and reverse primers.

MOL9771

100 µL

FACTOR V LEIDEN MUTANT PRIMER PAIR

For amplification and detection of the Leiden (G1691A) mutation with a CFR610 labeled forward primer.

MOL9050

50 µL

FACTOR V WILD TYPE PRIMER PAIR

For amplification and detection of the wild type sequence surrounding nucleotide 1691 with a FAM labeled forward primer.

MOL9051

50 µL

MTHFR 677 HYBEACON GEN II

For amplification and detection of the sequence that surrounds nucleotide 677 of the human methylenetetrahydrofolate reductase gene with a CFR610 labeled probe, forward and reverse primers.

MOL9775

100 µL

MTHFR 677 MUTANT PRIMER PAIR

For amplification and detection of the C677T mutation with a CFR610 labeled forward primer.

MOL9054

50 µL

MTHFR 677 WILD TYPE

For amplification and detection of the wild type sequence surrounding nucleotide 677 with a FAM labeled forward primer.

MOL9055

50 µL

MTHFR 1298 HYBEACON GEN II

For amplification and detection of the sequence that surrounds nucleotide 1298 of the human methylenetetrahydrofolate reductase gene with a Q670 labeled probe, forward and reverse primers.

MOL9777

100 µL

MTHFR 1298 MUTANT PRIMER PAIR

For amplification and detection of the 1298C mutation with a Quasar 670 labeled forward primer.

MOL9056

50 µL

MTHFR 1298 WILD TYPE PRIMER PAIR

For amplification and detection of the wild type sequence surrounding nucleotide 1298 with a JOE labeled forward primer.

MOL9057

50 µL

PROTHROMBIN (FII) HYBEACON GEN II

For amplification and detection of the sequence that surrounds nucleotide 20210 of the human prothrombin (factor II) gene with a CFR610 labeled probe, forward and reverse primers.

MOL9773

100 µL

PROTHROMBIN (FII) MUTANT PRIMER PAIR

For amplification and detection of the Prothrombin (Factor II) G20210A mutation with a Quasar 670 labeled forward primer.

MOL9052

50 µL

PROTHROMBIN (FII) WILD TYPE PRIMER PAIR

For amplification and detection of the Prothrombin (Factor II) wild type sequence surrounding nucleotide 20210 with a JOE labeled forward primer.

MOL9053

50 µL

Parasite

Product
Descrizione
Codice
Volume/Quantity/Reactions
PLASMODIUM PRIMER PAIR

For amplification and detection of a well conserved region of the mitochondrial cytochrome c oxidase III (cox3) gene for Plasmodium species with a FAM labeled probe, forward primer, and reverse primer.

MOL9065

25 µL

T. GONDII PRIMER PAIR

For amplification and detection of the B1 gene with a FAM labeled forward primer.

MOL9025

50 µL

Fungal

Product
Descrizione
Codice
Volume/Quantity/Reactions
C. AURIS PRIMER PAIR

For amplification and detection of a well conserved region of the internal transcribed spacer 2 (ITS2) region in the rRNA gene for Candida auris with a FAM labeled probe, forward and reverse primer.

MOL9059

25 μL

P. JIROVECII PRIMER PAIR

For amplification and detection of the mtLSU gene with a CFR 610 labeled forward primer.

MOL9098

25 µL

Bacterial

Product
Descrizione
Codice
Volume/Quantity/Reactions
A. PHAGOCYTOPHILUM PRIMER PAIR

For amplification and detection of the GroEL gene for Anaplasma phagocytophilum with a FAM labeled probe, forward and reverse primer.

MOL9062

25 µL

B. HOLMESII PRIMER PAIR

For amplification and detection of hIS1001, Bordetella holmesii insertion sequence, with a JOE labeled forward primer.

MOL9036

50 µL

B. PARAPERTUSSIS PRIMER PAIR

For amplification and detection of the IS1001 insertion sequence with a CAL Fluor Red 610 labeled forward primer.

MOL9007

MOL9307

50 µL

200 µL

B. PERTUSSIS PRIMER PAIR

For amplification and detection of the IS481 insertion sequence with a FAM labeled forward primer.

MOL9006

MOL9306

50 µL

200 µL

BABESIA PRIMER PAIR

For amplification and detection of a well conserved region of the 18S rRNA gene for Babesia species with a JOE labeled probe, forward and reverse primer.

MOL9063

25 µL

BARTONELLA PRIMER PAIR

For amplification and detection of a well conserved region of the ribosomal small subunit methyltransferase H gene (rsmH) for Bartonella species with a FAM labeled probe, forward primer, and reverse primer.

MOL9066

25 µL

BORRELIA PRIMER PAIR

For amplification and detection of the flaA gene gene with a FAM labeled forward primer.

MOL9015

MOL9315

50 µL

500 µL

C. DIFFICILE PRIMER PAIR

For amplification and detection of the toxin B gene with a FAM labeled forward primer.

MOL9026

50 µL

C. PNEUMONIAE PRIMER PAIR

For amplification and detection of the Cpn0980 gene with a FAM labeled forward primer.

MOL9023

50 µL

C. PNEUMONIAE II PRIMER PAIR

For amplification and detection of the Cpn0980 gene with a JOE labeled probe, forward and reverse primer.

MOL9400

25 µL

EHRLICHIA PRIMER PAIR

For amplification and detection of a well conserved region of the 16S rRNA gene for Ehrlichia species with a CAL Fluor Red 610 labeled probe, forward and reverse primer.

MOL9064

25 µL

GROUP A STREP PRIMER PAIR

For amplification and detection of exotoxin B (spe B) with a FAM labeled forward primer.

MOL9033

MOL9333

MOL9333-5

50 µL

1000 µL

5 x 1000 µL

GROUP B STREP PRIMER PAIR

For amplification and detection of CFB gene with a CFR(610) labeled forward primer.

MOL9047

50 µL

GROUP C STREP PRIMER PAIR

For amplification and detection of CPN gene with a CFR(610) labeled forward primer.

MOL9093

50 µL

GROUP G STREP PRIMER PAIR

For amplification and detection of mrcB gene with a JOE (560) labeled forward primer.

MOL9094

50 µL

KPC PRIMER PAIR

For amplification and detection of the Klebsiella pneumoniae carbapenemase gene, also known as blaKPC, with a FAM labeled forward primer.

MOL9035

50 µL

L. PNEUMOPHILA PRIMER PAIR

For amplification and detection of the pmiA gene with a CFR610 labeled forward primer.

MOL9034

50 µL

LEGIONELLA PRIMER PAIR

For amplification and detection of a well conserved region of the 16S rRNA gene of Legionella species with a CFR610 labeled probe, forward and reverse primers.

MOL9401

25 µL

M. PNEUMONIAE PRIMER PAIR

For amplification and detection of the P1 gene with a FAM labeled forward primer.

MOL9013

50 µL

M. PNEUMONIAE II PRIMER PAIR

For amplification and detection of a well conserved region of the P1 gene for Mycoplasma pneumoniae with a FAM labeled probe, forward primer and reverse primer.

MOL9058

25 µL

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