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xMAP® Applications

Immunoassays and nucleic acid assays

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xMAP® Technology supports both immunoassays and nucleic acid applications.

Tecnologie xMAP® per Test Immunologici e dell'acido nucleico - Diasorin

xMAP® Technology is commonly used for:

  • Biomarker characterization
  • Identification of secreted proteins
  • Mechanism of action studies
  • Patient stratification
  • Therapeutic drug monitoring
  • Gene expression analysis
  • Single nucleotide polymorphism (SNP) detection
  • Pathogen detection and typing
  • Exosome studies
  • MicroRNA analysis

xMAP® in action

Explore xMAP® Technology and discover some of the many applications used
by multiplexing research experts across the globe.

APPLICATIONS

Bead-Based Multiplexing vs. Electrochemiluminescence

Bead-based multiplexing offers significantly higher plex capacity, requires less hands-on time and delivers superior results compared to traditional methods.

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APPLICATIONS

xMAP® Assay Automation

Automation can boost productivity, minimize errors, and save on reagents.

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APPLICATIONS

Immunogenicity

By using xMAP® Technology, researchers can consolidate multiple assays into one for more efficient immune response studies.

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Immunoassays

For immunoassays, the capture sandwich format is commonly used for xMAP® protein assays, where the analyte is “sandwiched” between a capture and detection antibody. The capture sandwich format is compatible with a wide variety of target analytes.

A competitive format is used when the target analyte has a low molecular weight and only one or two antibody-binding epitopes or if only one antibody is available. Competitive xMAP® assays can be developed using either antibody-coupled or antigen-coupled beads and can be multiplexed with the capture sandwich format in the same assay.

An indirect format is used for serological assays to measure the amount of antigen-specific antibody present in biological samples. Once a format has been chosen, the beads can be coupled to the proteins used in the assay.

Capture Sandwich Format per Test Immunodiagnostici - Diasorin

Nucleic Acid Assays

The open architecture of xMAP® Technology enables numerous nucleic acid assay applications. Multiplex nucleic acid assays are developed by coupling user-defined sequence-specific capture oligos to MagPlex® beads or using MagPlex®-TAG™ beads that are already coupled to unique 24-base DNA sequences (anti-TAGs).

Tecnologia xMAP® per test sul rilevamento di acidi nucleici - Diasorin

What can be coupled to beads

Tecnologie xMAP® per Test Immunologici e dell'acido nucleico - Diasorin

xMAP® Assay Support

Looking for custom assay design and development services with unique targets in mind? Discover the xMAP® Assay Support and partner with the xMAP® multiplexing industry experts.

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xMAP® Kit Finder

xMAP® Technology is an open multiplexing platform used in hundreds of commercial immunoassay kits. Industry leading manufacturers offer xMAP® Technology assay kits. To find them, use our xMAP® Kit Finder.

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