Technology Overview
Generate more data while saving sample, time, and reagents
xMAP® Technology is the leading multiplexing platform for high-throughput testing, enabling simultaneous testing of up to 500 analytes in a single run. Combining efficiency and flexibility, xMAP® Technology allows you to generate more data while conserving sample, time, and reagents.
Supporting both immunoassays and nucleic acid-based tests, xMAP® Technology is trusted for a wide range of research and clinical applications, including biomarker characterization, gene expression analysis, pathogen detection, and more.
Explore how XMAP® Technology works and discover how our unique bead-based multiplex platform can accelerate your research with reliable results.
xMAP® Technology
The world’s most used multiplexing technology
Multiplexing is a method of high-volume biomarker testing—or testing multiple analytes simultaneously within a single run—using a single sample volume. xMAP® Technology is best suited to perform a wide range of protein- and nucleic-acid based multiplex assays, and can test anywhere from three to 500 analytes at once.
xMAP® means Multi-Analyte Profiling, where the “x” represents the biomarker (such as proteins, nucleic acids, or polysaccharides) that is being measured.
How xMAP® Technology works
xMAP® Technology uses labeled microspheres or beads, allowing for the simultaneous capture of multiple analytes from a single reaction. Because of their small size and low density, xMAP® Microsphere-based assays exhibit virtual solution-phase kinetics during the reaction. The beads are individually read using an xMAP® instrument. When testing between three and 500 targets, xMAP® Technology saves sample, time, and reagents compared to singleplex techniques.
xMAP® Technology benefits
High-throughput testing and analysis
Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously.
Versatility
A single xMAP® system can perform assays using several different methods and chemistries, including nucleic acid hybridization, antigen-antibody binding, enzyme activity, receptor-ligand binding, and other protein interactions.
Accuracy
xMAP® Technology generates data and accurately quantifies the biological interactions being tested.
xMAP® Microspheres
xMAP® Microspheres (beads) pass through a red laser or LED, which excites the internal dyes to distinguish the microsphere set. Then, a green laser, green LED, or violet laser (xMAP® INTELLIFLEX DR-SE only*) excites the fluorescent reporter dye to determine the result of the assay.
*For Research Use Only. Not for use in diagnostic procedures.
xMAP® applications
xMAP® Technology supports both immunoassays and nucleic acid applications. Explore xMAP® Technology and discover some of the many applications used by multiplexing research experts across the globe, including but not limited to: biomarker characterization, gene expression analysis, therapeutic drug monitoring, and exosome studies.
xMAP® instruments
Multiplexing solutions for proteins and nucleic acids.
Reagents and accessories
Different bead types, reagents, and other accessories are available to produce the best results.
Luminex LTG Partners
Accelerate your research with leading multiplexing industry experts and solution providers.
Microspheres
Discover the power behind xMAP® Technology. Our xMAP® Microspheres enable the development of accurate, efficient, and reliable multiplex assays.
xMAP® Kit Finder
Browse top-quality assays from trusted Partners. Quickly locate kits tailored to your needs with our easy-to-use search tool.
xMAP® Cookbook
Explore expert methods and protocols to design, optimize, and validate your multiplex assays. Streamline your workflow and maximize results with xMAP® Technology.
White Papers
Access expert insights, practical guidance, and proven strategies to enhance your multiplex assays.
Videos
Watch tutorials, expert interviews, and webinars. Explore our video library for practical tips and insights to advance your research.