MagPlex® Monitoring Microspheres
Monitor assay and instrument performance
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About
MagPlex® Monitoring Microspheres are magnetic polystyrene beads that provide insight into factors impacting target median fluorescent intensity (MFI) values, including instrument fluctuations and user error.
All microspheres are general-use components for further manufacture of xMAP® bioassays.
Features
- Magnetic Microspheres: MagPlex® Monitoring Microspheres are magnetic polystyrene microparticles that have been color-coded to classify to a specific region.
- Additional reporter dye: In addition to the classification dyes, MagPlex® Monitoring Microspheres contain an internal reporter dye that generates reporter median fluorescent intensity (MFI) values specific to instrument-type and reporter modes.
- Microsphere interrogation: The MagPlex® Monitoring Microsphere is excited by the optical system of the xMAP® Instrument, and resulting MFIs can be analyzed for indications of performance errors.
Benefits
- Increased confidence in your results: Monitor well-to-well assay performance for errors, ensuring each individual well has not been impacted by external factors.
- Broad application areas: These microspheres can be incorporated into various assay formats, including proteomic and genomic applications.
- The ability to pinpoint errors quickly: MFI values generated using these microspheres can eliminate instrument and user errors as a source of unexpected results.
Workflow
MagPlex® Monitoring Microspheres can be incorporated into new or existing assays.
Incorporate MagPlex® Monitoring Microspheres into bead mix
After assay validation, incorporate MagPlex® Monitor beads into the assay bead mix.
Perform assay steps as normal.
Analyze samples
Analyze each sample well, containing both the assay and monitoring beads, on an xMAP® instrument.
Review results
Compare results to validated assay thresholds to verify there are no user errors or instrument malfunctions.
Performance
| |
MagPlex® RP1 Monitor |
MagPlex® RP2 Monitor |
|---|---|---|
Magnetic |
Yes |
Yes |
Assigned Region |
045 |
222 |
RP1 MFI Value Range |
MAGPIX® Luminex® 200™ FLEXMAP 3D® xMAP INTELLIFLEX® Systems FLEXMAP 3D® Mode xMAP INTELLIFLEX® Mode |
N/A |
RP2 MFI Value Range |
N/A |
xMAP INTELLIFLEX® DR-SE |
Instrument Compatibility |
MAGPIX® |
xMAP INTELLIFLEX® DR-SE* |
| *For research use only. Not for use in diagnostic procedures. |
To be used on
Multiplexing systems designed for ease of use, flexibility, and scalability to enable custom assay development for protein and nucleic acid-based multiplex testing.
xMAP INTELLIFLEX® System
The only compact, flow-based multiplex platform that combines the proven xMAP® Technology with modern features to enhance performance, empower innovation, and simplify your user experience.
Luminex® 200™ System
A fast, cost-effective, flow-based analyzer that simultaneously measures up to 100 analytes (on MicroPlex Microspheres) or
80 (on MagPlex Microspheres) in a single microplate well.
MAGPIX® System
A fast, compact, cost-effective, imaging-based analyzer that simultaneously measures up to 50 analytes in a single microplate well.
MagPlex® Monitoring Microspheres ordering info
Product name |
Volume |
Concentration (microspheres/mL) |
Region |
Registration status |
Part number |
|---|---|---|---|---|---|
MagPlex® RP1 Monitor |
1 mL |
1.25 x 107 |
45 |
For Further Manufacture |
MRP1-045-01 |
MagPlex® RP2 Monitor |
1 mL |
1.25 x 107 |
222 |
For Further Manufacture |
MRP2-222-01 |
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Delivering the versatility to perform a wide range of protein and nucleic acid-based multiplex assays—all using xMAP® Technology.