xMAP® Technology
The world’s most used multiplexing technology
Multiplexing is a method for high-volume biomarker testing—or testing multiple analytes simultaneously within a single run—using a single sample volume. xMAP® Technology is best suited for testing 3–500 targets.
xMAP® Technology allows users to perform a wide range of protein- and nucleic acid-based multiplex assays, which can simultaneously detect up to 500 targets in a single run.
xMAP means Multi-Analyte Profiling, where the “x” represents the biomarkers (such as proteins, nucleic acids, or polysaccharides) that are being tested.
How does xMAP Technology work?
xMAP® Technology uses labeled microspheres or beads, allowing for the simultaneous capture of multiple analytes from a single reaction. Because of their small size and low density, xMAP® microsphere-based assays exhibit virtual solution-phase kinetics during the reaction. The beads are individually read using an xMAP® instrument.
When testing between 3 and 500 targets, xMAP® Technology saves sample, time, and reagents compared to singleplex techniques.
Generate more data while saving sample, time, and reagents.
xMAP® Beads
xMAP® beads pass through a red laser, or LED, which excites the internal dyes to distinguish the microsphere set.
Then, a green laser or LED excites the fluorescent reporter dye to determine the result of the assay.
xMAP® Technology benefits
High-throughput testing and analysis
Because each microsphere serves as an individual test, a large number of different assays can be performed and analyzed simultaneously.
Versatility
A single xMAP® system can perform assays using several different methods and chemistries, including nucleic acid hybridization, antigen-antibody binding, enzyme reactivity, receptor-ligand binding, and other protein interactions.
Accuracy
xMAP® Technology generates data and accurately quantifies the biological interactions being tested.
Instruments based
on xMAP® Technology
Multiplexing solutions for proteins and nucleic acids.
Reagents and
accessories
Different bead types, reagents and other accessories are available to produce the best results.
xMAP® applications
Explore xMAP® Technology and discover some of the many applications used by multiplexing research experts across the globe.
LuminexPLORE Lab
Looking for custom assay design and development services with unique targets in mind? Discover the LuminexPLORE Lab and partner with the xMAP® multiplexing industry experts.
xMAP® Kit Finder
xMAP® Technology is an open multiplexing platform used in hundreds of commercial immunoassay kits. Industry leading manufacturers offer xMAP® Technology assay kits. To find them, use our xMAP® Kit Finder.