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Luminex® 200 System

Multiplex up to 100 analytes in a single well of a microtiter plate

The Luminex® 200 System is a  fast, robust, flow-based analyzer combining three core xMAP® Technologies: the fluorescently dyed micron-sized polystyrene microspheres acting as both the identifier and the solid surface to build the assay; a flow cytometry-based instrument, integrating key xMAP® detection components, and the xPONENT® Software, which is designed for protocol-based data acquisition with robust data regression analysis.

LUMINEX® 200™ Instrument System - Diasorin
Utilizzo del Sistema Luminex® 200™ - Diasorin
01

Flexibility

Choose from thousands of commercially-available kits, or develop custom assays.

Luminex Flow Based Analyzers - Diasorin
LUMINEX® 200™ Instrument System - Diasorin
02

Speed

With a read time of ≤45 min per 96-well plate and the ability to multiplex up to 100 analytes per reaction, Luminex® 200 can provide up to 12,800 results per hour.

LUMINEX® 200™ Multiplex Analyzers - Diasorin
LUMINEX® 200™ Instrument System - Diasorin
03

Reliability

The workhorse of the xMAP® multiplexing family is easy to use and maintain, consistently providing fast and reliable results.

Expert Using Diasorin LUMINEX® 200™ System - Diasorin
LUMINEX® 200™ Instrument System - Diasorin

Why choose Luminex® 200 System

A robust and reliable multiplexing system with more than 9,000 citations.

01

Genomic and proteomics drug discovery

  • Single nucleotide polymorphisms (SNPs)
  • Expression analysis
  • High-throughput compound screening
  • Kinase selectivity screening of candidates
  • Serological studies
  • Multianalyte drug and drug metabolite profiling
  • Toxicology & drug metabolism studies
  • Drug target validation (receptor-ligand analysis)
02

Diagnostic applications

  • Genetically inherited disease screening (e.g., cystic fibrosis)
  • HLA typing for transplantation
  • Autoimmune disease profiling
  • Molecular pathogen detection
03

Research assay applications

  • Gene expression analysis
  • Genotyping
  • Protein expression analysis
  • Animal-model serum analyte profiling
  • Antibody epitope mapping
  • Enzyme/substrate research
  • Protein-protein interaction analysis

System

Product
Registration status
Part number
Luminex® 200 System with xPONENT® 4.3

IVD

RUO

LX200-XPON-IVD

LX200-XPON-RUO

Software

Product
Description
Part number
21 CFR Part 11

For laboratories working under the FDA’s 21 CFR Part 11 regulations, xPONENT® offers multilevel user management, full audit trail, electronic records, and electronic signatures.

XPON-21CFR

Automation Connectivity Module

The automation module along with a device driver enable communication between the reader and a front-end automation platform.

XPON-AUTO

LIS Connectivity Module

This module allows communication with an external Laboratory Information System (LIS) database by allowing the export of patient result data and the import of patient sample information in ASTM format; connection to your LIS system may require the use of middleware.

XPON-LIS

Luminex® 200™ xPONENT® 4.3 IQ/OQ Protocol

Installation Qualification and Operational Qualification protocols provide documented evidence that the instrument has been installed and is operating according to Luminex guidelines and specifications.

VP-LX-XPON-IQOQ

Security Module

The secure xPONENT® package includes all the basic functionality, plus allows an administrator to set up user access levels.

XPON-SECURITY

PC with xPONENT® 4.3 Pre-installed

xPONENT® software provides the basic functionality required for instrument control, data acquisition, and data reduction. xPONENT® 4.3 is available as pre-installed software on a Luminex-supplied PC.

XPON-UPGRD-LX

Accessories & spare parts

Product
Part number
6-Month Preventative Maintenance Kit

CN-0096-01

Automated Maintenance Plate

CN-0206-01

Barcode Scanner, USB

CN-PC03-01

Heater Block (XYP)

CN-0017-01

Intake Air Filter

CN-0027-01

Long Sample Probe

CN-0007-01

Rear Air Filter

CN-0001-01

Sample Needle Height Alignment Kit

CN-0015-01

Sheath Filter w/ Quick Disconnect

CN-0010-01

Syringe Cylinder, w/ Seal

CN-0013-01

Reagents

Product
Registration status
Part number
Luminex® 200 (xPONENT® ONLY) Calibration Kit (25 uses)

IVD

RUO

LX200-CAL-K25

LX2R-CAL-K25

Luminex® 200 (xPONENT® ONLY) Performance Verification Kit (25 Uses)

IVD

RUO

LX200-CON-K25

LX2R-PVER-K25

xMAP® Sheath Fluid PLUS

IVD

RUO

40-50035 40-50021

xMAP® Sheath Concentrate PLUS

IVD

RUO

40-50036 40-50023

Specifications

Product Details

General

Physical dimensions

Luminex® 200: 43 cm (17 in.) x 50.5 cm (20 in.) x 24.5 cm (9.5 in.) (W x D x H)
Luminex XYP: 44 cm (17.25 in.) x 60 cm (23.5 in.) x 8 cm (3 in.) (W x D x H)
Luminex SD: 20 cm (8 in.) x 30 cm (11.75 in.) x 24.75 cm (9.75 in) (W x D x H)


Note: Allow an additional 3.18 cm (1.25 in.) clearance to all dimensions for proper cooling

Weight

Luminex® 200: 25 kg (60 lbs)
Luminex XYP: 15 kg (33 lbs)
Luminex SD: 9 kg (20 lbs)

Operating Temperature

15°—30°C (59°—86°F)

Operating Humidity

20—80%, non-condensing

Shipping and Storage Temperature

0—50°C (32°—122°F)

Shipping and Storage Humidity

20—80%, non-condensing

Altitude

Operation up to 2,400 m (7,874 ft.) above mean sea level

System Warmup Time

30 min
Systems that remain inactive for at least four hours will require a warmup to restart the lasers. The system resets the four-hour internal clock after acquiring the sample, running system calibrators, running system controls, or warming up the instrument.

System Initialization

<45 min. (Including laser warmup and weekly calibration)

System Verification

5 min

Heater Operating Range

Maintains samples using the heater block (35—60°C +/- 2°C of set point; 95—131°F) 

Plate Run Time

96-well in ≤45 min (up to 12,800 tests/hour)

Multiplexing capability

Up to 100 analytes per sample (80 on MagPlex® microspheres)

Electronics

Input Voltage Range

100—120 V~ ±10%, 1.4 Amp, and 200—240 V~ ±10%, 0.8 Amp, 47—63 Hz

Installation Category

II - As defined in IEC 61010-1:2010

Pollution Degree

2 - As defined in IEC 61010-1:2010

Optics

Reporter Channel Detection

A/D resolution 14 bits

Reporter Channel Dynamic Range

≥ 3.5 decades of detection

Reporter Laser

532 nm, nominal output 10 mW—15 mW maximum 500 mW, frequency-doubled diode; mode of operation, continuous wave (CW)

Classification Laser

635 nm, 9.1 ± 6%, maximum output 25 mW, diode; mode of operation, continuous wave (CW)

Reporter Detector

Photomultiplier tube, detection bandwidth of 565—585 nm

Classification Detector

Avalanche photodiodes with temperature compensation

Doublet Discrimination Detector

Avalanche photodiodes with temperature compensation

Fluidics

Cuvette

200 micron square flow channel

Sample Injection Rate

1 μL/second

Sample Uptake Volume

20—200 μL

Sheath Flow Rate

5.4 (± 0.3) mL/min

Sheath Pressure

6—9 PSI for normal operations

PC and Monitor Specifications

Processor

3.0 GHz Intel Core i5 (or higher)

Main Memory

8 GB RAM

Hard Drive

1 TB Hard drive space (or higher)

Ports

1x USB 3.1 Type-C
5x USB 3.1 Type-A
4x USB 2.0 Type-A
2x Display ports
1x VGA port
1x DVD R/W type drive

Operating System

Microsoft® Windows® 10 Professional 64 bit, version 1709 or greater

Screen Resolution

SXGA 1,280 x 1,024, 32 bit color

Screen Size

48.3 cm (19 in.)

Display Settings

96 DPI Font
Default Windows® 10 theme

Regulatory

All Luminex instruments are marked in compliance with electrical/safety device standards for appropriate regions. For details on approvals and standards compliance please contact Luminex.

IQ/OQ Services

Installation Qualification (IQ) and Operational Qualification (OQ) services are performed by Luminex Field Service Engineers, and are critical to ensuring that your instrument is properly installed and operating according to specifications. For more information, please click here, contact your Luminex representative, or email orders@luminexcorp.com.

More details about Luminex® 200 System

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